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[ - Advanced Lesson]
Objectives
Students will be able to:
1. Create a piece of double stranded DNA that encoded for a known protein.
2. Clearly distinguish between coding and template strands of DNA.
3. Understand the relationship of mRNA to the coding and template strands of DNA.
mAteriAls
It is recommended that four* student groups work together for this exercise. They should have
the following:
• 4kitsfromtheK’NEX DNA, Replication and Transcription Class Pack containing 20 foot flexible
tubing (recommended)
OR
4 K’NEX DNA, Replication and Transcription kits
• 1buildinginstructionsbookletpergroup
• Studentjournals(1perstudent)
• CopiesoftheGeneticCodeTable(Table1below)
• CopiesoftheAminoAcidSequenceforGlucagon(Figure1below)
* If there are fewer than 4 groups, these should work to create as many glucagon segments as
possible, creating a partial molecule.
MODULE III: CODING, TRANSLATION, AND MUTATIONS
Table 1 – The Genetic Code: the three-letter codes representing the possible sequences of nitrogen-containing
bases found in mRNA to represent the 20 amino acids and stop codons.
[Source for Table 1: Fig. 5.6, p.143 from BIOCHEMISTRY, 3rd ed. by Christopher K. Mathews, K.E. van Holde and Kevin G. Ahern. Copyright
©
2000
by Addison Wesley Longman, Inc. Reprinted by permission of Pearson Education, Inc.]
888-ABC-KNEX
Education
®
35


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